DNA Testing Requirements

The following information relates to DNA testing requirements for registration with Dilutes Australia Ltd.

Please read these prior to completing and lodging any paperwork for registration with the company.

More information can be obtained by contacting either the Registrar (dilutes@iinet.net.au) or the General Secretary (pgrossington@bigpond.com)


All DNA results will be recorded on registration certificates.

What must be tested....

All solid dilutes with a grey parent must be DNA'd for grey (UNLESS the owner states the horse or pony carries grey) and must return a negative test result to be eligible for registration .

All solid dilutes with a roan parent must be DNA'd for roan where a roan test is available for that breed (UNLESS the owner states the horse or pony carries roan) and must return a negative test result to be eligible for registration.  Breeds for which a roan DNA test is not available will be required to be tested as soon as tests become available and if found to be positive, will be de-registered.

Black and brown buckskins:

All horses / ponies being presented for registration as either a black or a brown buckskin will be required to undergo DNA testing for the cream factor if it is determined by the registrar that there is not sufficient physical evidence of dilution. Sufficient evidence includes any one or more of the following criteria:

  • proof of double CREAM dilute parentage,
  • production of CREAM dilute progeny from non-cream dilute partners
  • double CREAM dilute progeny
  • obvious dilute foal colour (many black buckskin foals are clearly dilutes at birth but this may not be so apparent as they age)

Cremellos and perlinos:

All cremellos and perlinos with unknown parentage must be DNA'd for grey and roan (if available) and must return negative test results to be eligible for registration. They may additionally be required to be DNA tested for all available coat pattern genes (OLWS, tobiano, splash, Lp etc) if there is cause to suspect that they may be exhibiting some of these coat patterns.

Duns:

All horses and ponies being presented for dun registration that exhibit primitive markings which in the opinion of the registrar are not consistent with those found on true genetic duns will be required to undergo a dun zygosity test unless they have a proven homozygous dun parent.

Silvers:

All horses and ponies being presented for registration as Silvers which do not have a proven homozygous silver parent may be required to be DNA'd for the silver & / or agouti at the registrar's discretion.

Pearls:

Horses being presented for registration as heterozygous pearls on a base colour (red, bay, brown or black) which do not have a proven homozygous pearl parent will be required to undergo DNA testing for pearl.

Paint or pinto parentage:

Horses being presented for solid STUDBOOK dilute registration with REGISTERED PAINT OR PINTO PARENTAGE must be DNA tested for all currently available coat pattern gene tests.  Subsequent progeny of these horses and ponies will not be required to be DNA'd for coat pattern genes PROVIDING the other parent is also solid and is also negative for any coat pattern genes.  If they return a positive test for any coat pattern gene or testing is not done they will only be eligible for non-studbook registration provided they conform to the company's regulations with respect to white markings & eye colour.  Any solid dilute progeny from horses not tested or returning positive results for coat pattern genes will also be required to be tested for the same coat pattern genes before being eligible for studbook registration.

Horses being presented for solid NON-STUDBOOK dilute registration with UNREGISTERED PAINT OR PINTO PARENTAGE may be DNA tested for all currently available coat pattern gene tests at the owner's discretion but if not tested, OR if they return a positive result for any coat pattern gene, subsequent solid dilute progeny of these horses and ponies will be required to be DNA'd for coat pattern genes and must return a negative result to be eligible for studbook registration.

Solid dilutes with GENETIC PAINT OR PINTO PARENTAGE (WHETHER THE PATTERN IS EXPRESSED OR NOT) must be DNA tested for all currently available coat pattern genes (OLWS, tobiano etc) before they will be eligible for studbook registration.

Appaloosa, Palouse Pony, Spotted Pony parentage:

All solid dilute horses and ponies with REGISTERED APPALOOSA, PALOUSE PONY OR SPOTTED PONY PARENTAGE will be eligible for studbook registration upon the return of negative DNA tests for Lp provided they conform to the company's regulations with respect to white markings & eye colour. Subsequent progeny of these horses and ponies will not be required to be DNA'd for Lp PROVIDING the other parent is also solid and is also negative for Lp.

All solid dilute horses and ponies with UNREGISTERED APPALOOSA, PALOUSE PONY OR SPOTTED PONY PARENTAGE will be eligible for non-studbook registration upon the return of negative DNA tests for Lp provided they conform to the company's regulations with respect to white markings & eye colour. Subsequent progeny of these horses and ponies will not be required to be DNA'd for Lp PROVIDING the other parent is also solid and is also negative for Lp.

Any dilute with APPALOOSA, PALOUSE PONY OR SPOTTED PONY PARENTAGE that returns a positive test for Lp is only eligible for DA Broken Coloured Dilute registration provided they conform to the regulations for that register with respect to broken coloured breed society registration.

Unknown parentage - all dilute registers:

The progeny (and subsequent generations) of all mares and stallions with unknown parentage that have NOT been DNA tested for all currently available coat colour pattern genes, regardless of colour and register, will remain non-studbook UNTIL such time as the required DNA testing is carried out on any generation. If the tests are negative, the individual/s that have been tested and subsequent generations will be eligible for studbook registration.

Hardship Clause:

Solid dilutes being presented for studbook or non-studbook registration under the hardship clause must be tested for OLWS and Tobiano and must return negative results for both to be considered for acceptance under this clause.

Procedure for DNA collection and submission:

Suggested Labs

Please note that Dilutes Australia accepts DNA results from any accredited lab; the following labs are recommendations only due to their history of providing reliable results in a good turnaround time.

Practical Horse Genetics (AUS)

Please visit the website for information about lodging samples and prices.

Animal Genetics (US)

All forms are available from the lab's website: www.animalgenetics.us.

UC Davis (US)

Please visit the website for information about lodging samples and prices.


Procedures

We have also provided a more detailed guide in pdf format.

Step 1: download the DNA collection verification form (an authorised person includes a Dilutes Australia accredited classifier or nominated person or a branch committee member)

Step 2: download the DNA kit from the lab's website Instructions for collection are contained on this form. Tick the boxes for the required tests:

  • cream for black or brown buckskins
  • silver and both bay and brown agouti for silvers
  • grey as applicable
  • OLWS / tobiano / SW1 / SW2 / SW3 / LP as applicable
  • champagne etc
  • pearl

The roan and dun tests are only available through UC Davis.

Brown agouti (At) is only available through Pet DNA Services.

Step 3: Download the Import Permit if using one of the US labs (please note that this document must be included with all samples sent to the lab because if a random check is carried out at Customs and the permit is not included, the sample will be confiscated)

Step 4: complete both forms as per instructions contained on each.

Step 5: complete an Australian customs permit available from the post office if using an overseas lab.

Step 6: send to addresses as per instructions on each form.

Step 7: the lab will notify you by email of the results. Either attach a copy of the email to the registration applications OR forward by email to the registrar at dilutes@iinet.net.au


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